Ashwagandha Extract 1.5% by HPLC

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Ashwagandha Extract 1.5% by HPLC


Botanic Healthcare

Product  Name

Ashwagandha Extract 1.5% by HPLC 



Country of Origin



5 Kg To 1 Ton

Aroma Profile

Earthy aroma with a bitter taste

Batch Information

Extraction Method

Solvent Extraction

Botanical Name

Withania somnifera

Chemical Constituents

Contains Withanolides and Alkaloids

Color and Appearance

Brown colored powder

Plant Part Used




Available as per request


Available as per request

GC-MS Analysis

Available as per request

Container type

Sealed Container

Storage Recommendations

Store in a cool and dry place. Avoid exposure to light.


Ashwagandha extract 1.5% withanolides is manufactured under the high-performance liquid chromatography (HPLC) process in a series of exacting steps to ensure pure, potent, and consistent yield.

1. Raw material preparation: Fresh and of best quality Ashwagandha roots were procured and cleaned thoroughly for all sorts of extraneous materials. The roots are dried at an optimum temperature in a manner so that the drying process does not affect the integrity of its active constituents.

2. Extraction: Take the dried roots of Ashwagandha and then make its powder fine. Subject the roots of the plant to a suitable solvent (e.g., water, ethanol, or their mixture) for the extraction process. This step should be optimized carefully to a proper extent to reach the maximum extraction of the withanolides while extracting the least amount of undesired compounds.

3. Concentration and Standardization: The extract is then concentrated under reduced pressure to remove the solvent, getting either a thick paste or dry powder. Where necessary, the dilution or blending with inert carriers to this extract may be done to make the standardization content at 1.5% withanolides.

4. HPLC Analysis: Withanolide determination will be carried out using High-Performance Liquid Chromatography (HPLC), which measures the content exactly and should not be less than 1.5%. This method analyzes the extract to give exact quantifications and purity results of withanolides.

5. Drying and Milling: The extract of standardization is dried to a defined level of moisture content and milled to a defined particle size that ensures uniformity and ability in the final product.

6. Quality Control and Packaging: Much quality control is considered on the final product level to meet quality standards, i.e., HPLC analysis, microbial testing, and purity assessments. After approval, Ashwagandha Extract is filled into a container resistant to moisture in the environment and sealed air-tightly to avoid stability and increase shelf life.


  • Enhances general wellness and relief formulations.
  • Supports athletic performance and recovery.
  • Helps in restful sleep patterns.
  • Incorporated into creams for its anti-inflammatory properties.
  • Added to foods and beverages for wellness benefits.

Geographical Specifications

The variations of Withania somnifera variations in phytochemical composition, especially withanolide content, are relatively very high, which depends on the geographical growth conditions of the plant. Indeed, it is established that plants grown under higher altitude conditions and in regions with large thermal amplitude tend to develop high concentrations of these bioactive compounds. This variation is the reflection of the plant's dynamic response to environmental stress, hence further influencing therapeutic efficacy in the extract from it.

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